In the initial stage of Intracytoplasmic Sperm Injection (ICSI), the male partner is tasked with providing a semen sample. This can be accomplished by ejaculating into a sterile cup or through surgical sperm retrieval. Alternatively, sperm may be retrieved in advance and preserved through freezing for future use. Meanwhile, the woman undergoes the extraction of multiple eggs from her ovaries using a fine needle and ultrasound probe. Although generally painless, this procedure may cause mild discomfort.